Exosomes derived Plant Stem Cells Nano-sized interorganismal communication vesicles

What are plant- derived exosomes, how do they affect humen cells?

Plant exosomes, spherical particles approximately 30-150 nm in size, are enveloped by a nanolipid bilayer membrane and play a fundamental role in intercellular communication and molecular transport by carrying various molecules such as proteins, lipids, and nucleic acids [1,2]. The presence of bioactive molecules like proteins and secondary metabolites within plant-derived exosomes has made them a significant research subject in cosmetic and pharmaceutical fields. While research to understand the potential of exosomes continues, particularly with the widespread use of next-generation sequencing technologies, the nucleic acid composition of plant-derived exosomes has been comprehensively revealed. One of the most striking findings of these studies is the presence of small RNA sequences, including specifically identified microRNAs, within plant exosomes [3-5]. Another noteworthy finding from this research is the demonstration that miRNAs contained in plant-derived exosomes can bind to human messenger RNA (mRNA) and modulate gene expression in human cells [6-12]. For example, ginseng-derived exosomes have been shown to regulate gene expression in human keratinocyte cells, particularly genes related to dermatological homeostasis. These exosomes have been found to reduce the expression of MMP12, MMP13, and NOTCH3 genes associated with the cutaneous aging process, while increasing the expression of FGF12, HS3ST3A1, and LOX genes related to dermal regeneration. Additionally, a significant increase was observed in the expression levels of VIM, ELOVL3, and KRT1 genes involved in epidermal barrier and hydration mechanisms [13]. Another recent study has demonstrated that plant-derived exosomal miRNAs can target and modulate gene expression in mammalian cells. In these studies, miRNAs obtained from plant exosomes were found to trigger neuronal differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and restore neurological functions both in vitro and in vivo [6]. The totality of the current literature findings indicates that plant-derived exosomal miRNAs have significant potential in modulating human gene expression.

Exosomes derived plant stem cells; ProliCell® Technology

As ACTV Biotechnology, we have developed ProliCell® technology, incorporating cutting-edge methods, to enable the high-yield and sustainable production of plant-derived exosomes.

Utilizing our patent pending ProliCell® technology, we harnessed the effects of plant stem cells and their synthesized exosomes. This approach enabled us to produce exosomes with high yield and content while minimizing plant usage. Exosomes derived from plant stem cells both possess the inherent cell renewal activity of stem cells and, due to the small RNAs they contain, exhibit gene-regulating effects on human skin cells.

Utilizing our unique ProliCell® technology, we isolate exosomal components and small RNA molecules from plant stem cells to offer innovative, cutting-edge products to the dermocosmetics sector. You can explore our 11 original products developed on the basis of our ProliCell® technology, which harnesses the synergistic potential of plant stem cells, exosomes, and small RNAs, bringing an innovative approach to the dermocosmetics sector.Leveraging the synergistic potential of plant stem cells, exosomes, and small RNAs through our ProliCell® technology, we have developed 11 uniques, active ingredients that offer effective solutions to various cosmetic challenges. You can explore these active ingredients and consult with us on how they can be utilized in your projects.

Referanslar

1. Dad, H. A., Gu, T. W., Zhu, A. Q., Huang, L. Q., & Peng, L. H. (2021). Plant exosome-like nanovesicles: emerging therapeutics and drug delivery nanoplatforms. Molecular Therapy, 29(1), 13-31.
2. Cao, M., Diao, N., Cai, X., Chen, X., Xiao, Y., Guo, C.,  & Zhang, X. (2023). Plant exosome nanovesicles (PENs): green delivery platforms. Materials horizons.
3. Teng, Y., Ren, Y. I., Sayed, M., Hu, X., Lei, C., Kumar, A., & Zhang, H. G. (2018). Plant-derived exosomal microRNAs shape the gut microbiota. Cell host & microbe, 24(5), 637-652.
4. Teng, Y., Xu, F., Zhang, X., Mu, J., Sayed, M., Hu, X., & Zhang, H. G. (2021). Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12. Molecular Therapy, 29(8), 2424-2440.
5. Xiao, J., Feng, S., Wang, X., Long, K., Luo, Y. I., Wang, Y., & Li, M. (2018). Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables. PeerJ, 6, e5186.
6. Xu, X. H., Yuan, T. J., Dad, H. A., Shi, M. Y., Huang, Y. Y., Jiang, Z. H., & Peng, L. H. (2021). Plant exosomes as novel nanoplatforms for microRNA transfer stimulate neural differentiation of stem cells in vitro and in vivo. Nano letters, 21(19), 8151-8159.
7. Teng, Y., Xu, F., Zhang, X., Mu, J., Sayed, M., Hu, X., & Zhang, H. G. (2021). Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12. Molecular Therapy, 29(8), 2424-2440.
8. Urzì, O., Gasparro, R., Ganji, N. R., Alessandro, R., & Raimondo, S. (2022). Plant-RNA in extracellular vesicles: the secret of cross-kingdom communication. Membranes, 12(4), 352.
9. Qiu, F. S., Wang, J. F., Guo, M. Y., Li, X. J., Shi, C. Y., Wu, F., & Yu, C. H. (2023). Rgl-exomiR-7972, a novel plant exosomal microRNA derived from fresh Rehmanniae Radix, ameliorated lipopolysaccharide-induced acute lung injury and gut dysbiosis. Biomedicine & Pharmacotherapy, 165, 115007.
10. Yan, L., Cao, Y., Hou, L., Luo, T., Li, M., Gao, S., & Zheng, L. (2024). Ginger exosome-like nanoparticle-derived miRNA therapeutics: A strategic inhibitor of intestinal inflammation. Journal of Advanced Research.
11. Kalarikkal, S. P., & Sundaram, G. M. (2021). Inter-kingdom regulation of human transcriptome by dietary microRNAs: Emerging bioactives from edible plants to treat human diseases?. Trends in Food Science & Technology, 118, 723-734.
12. Leng, Y., Yang, L., Pan, S., Zhan, L., & Yuan, F. (2024). Characterization of blueberry exosome-like nanoparticles and miRNAs with potential cross-kingdom human gene targets. Food Science and Human Wellness, 13(2), 869-878.
13. Cho, J. H., Hong, Y. D., Kim, D., Park, S. J., Kim, J. S., Kim, H. M., & Cho, J. S. (2022). Confirmation of plant-derived exosomes as bioactive substances for skin application through comparative analysis of keratinocyte transcriptome. Applied Biological Chemistry, 65(1), 8.